Steam sterilization is carried out in an autoclave. The resulting pressurized steam has a high latent heat. This intense heat causes protein hydrolysis and coagulation, which kills microorganisms, spores, and viruses. Steam sterilization usually involves exposing the item to steam at 121°C for 15 to 30 minutes. As temperature and pressure increase, the time required to disinfect items can be greatly reduced.

Steam sterilization is a more effective method than dry heat sterilization because the moisture in steam is a good conductor of heat and has an advantage in penetrating load. Since less energy is required, steam sterilization provides higher productivity and lower energy consumption, resulting in cost savings.
YG-P3 Biosafety Cabinet Steam Autoclave, which uses the effect of high pressure Steam and latent heat in the Steam environment as well as good penetration power, to coagulate and denature the bacterial protein and cause the death of microorganisms. It is suitable for sterilization of cloth work clothes, all kinds of utensils, metal instruments, rubber plug, distilled water, cotton plug, paper and some culture liquid. The steam pressure of the high-pressure steam sterilizer is generally adjusted to 1.0-1.1kg /cm2 and maintained for 20-30min to achieve the sterilization effect.
Dry heat sterilization is a method to kill bacteria and spores by using the high heat of 120°C ~150°C in a constant temperature drying oven and keeping it for 90-120 minutes.
Dry heat also kills bacteria to achieve the purpose of sterilization, but it is less efficient and is mainly suitable for sterilization that is inconvenient to be carried out in a pressure steam sterilizer, making it a less attractive option for most laboratories. Liquids are not compatible with dry heat sterilization because they boil if exposed to dry heat. Growth medium, flammable materials are also not suitable for dry heat sterilization. However, hydrophobic items such as fats and oils require dry heat, as well as items that can be damaged by moisture, such as powders; And instruments that may corrode.
Steam sterilization is the most widely used method with the best effect in laboratory at present. At present, the sterilization instrument is also very mature in technology and has high safety coefficient.