Choosing 4 Mushroom Substrate Recipes


Mushroom substrate recipes are varied and different combinations of recipes will be chosen depending on the type of mushroom, its stage of growth, and the purpose of cultivation. A few common mushroom substrate recipes are listed below:


Mushroom substrate recipe, mushroom media, culture medium


Wood Chip Medium:


-Wood shavings (about 75%-80% of total weight): sterilised at high temperature to ensure that there are no stray bacteria, generally choose wood shavings of broadleaf species that are moderately hard and have more sparse wood, such as basswood, poplar, willow or oak.


-Gypsum (about 1-2% of total weight): provides CaSO₄, which helps to regulate the pH of the medium between 5.5 and 6.5, while providing some mineral nutrients.


-Wheat bran or cornflour (about 15-20% of the total weight): serves as a source of nitrogen and other micronutrients, providing essential nutrients for mushroom mycelium growth.


-Water: added in moderation to keep the medium moist but not too wet, usually with a water content of about 60%-65%.


Straw Medium:


-Straw (about 60-70% of total weight): after crushing or milling, can be rice straw, wheat straw, corn stalks, etc., also need to be autoclaved.


-Wheat bran or maize flour (about 15-20% of the total weight): as an additional nutrient supplement, to ensure that the medium has enough nutrients for mycelium growth.


-Gypsum (approx. 1-2% of total weight): as above, to regulate pH and provide minerals.


-Water: add in appropriate amount to bring the water content of the medium to a suitable level.


Cow Dung Medium:


-Dried cow dung (about 60%-70% of total weight): pre-composted and dried to ensure freedom from pests and germs.


-Straw (approx. 10-20% of total weight): increases aeration and assists in providing a carbon source.


-Bran or maize meal (about 10-15 per cent of total weight): provides an additional source of nitrogen and other nutrients.


-Gypsum (approx. 1-2% of total weight): regulates pH.


-Water: moisten appropriately to ensure that the compost is moist but not waterlogged.


Bran Medium:


-Wheat bran (about 70%-80% of total weight): provides a rich source of carbon and nitrogen, suitable for the mycelial growth of certain types of mushrooms.


-Gypsum (about 1-2% of total weight): regulates pH and provides necessary mineral elements.


-Water: add water in appropriate amount and mix well to make it wet but not dripping.


The above formula often needs to be adjusted according to specific mushroom species, climatic conditions, seasonal changes and other factors in practical application, e.g. it may be necessary to increase the moisture-retaining ability of the medium when the temperature is low, and it may be necessary to appropriately increase the ventilation during the hot season. Meanwhile, different mushrooms have different nutritional preferences and growth habits, so it is also necessary to refer to the characteristics of specific strains of mushrooms for the selection of culture medium and optimisation of the ratio.


It is worth noting that sterilization is a very critical step in the production of culture medium to ensure that the medium does not contain any harmful microorganisms that may affect the normal growth of mushrooms. For this reason, our company specialises in manufacturing highly efficient and reliable sterilizers to provide users with one-stop sterilisation solutions. Our sterilization equipment adopts advanced technology, which is able to provide comprehensive and uniform high-temperature sterilization treatment to the culture medium, effectively killing potential pathogens and stray bacteria, providing strong support and guarantee for your mushroom growing business. View Our Solution Now.


Whether it is wood chip medium, straw medium or cow dung or wheat bran medium, our steriliser ensures that it is produced in a safe and sterile environment, thus greatly improving the quality of mushroom growth and yield.


After preparing the culture medium, it is usually put into plastic bags, bottles and jars or special mushroom culture containers, and then inoculated with mushroom strains for constant temperature and humidity cultivation.

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