The high pressure vapor sterilizer reaches a sterile requirement after sterilization of the item, which is the basic guarantee of microbial experiments. Is the high-pressure sterilizer sterilization effect is the most important top priority for the success or failure of the experiment. In addition to heating dissolution in addition to a few medium, most of the medium required for high pressure in high pressure, 20-30 minutes. This sterilization method is based on the boiling point of water as the principle of elevated steam pressure. When the steam pressure reaches 1.05 kg / cm 2, the temperature of the water vapor rises to 121 ° C, 15 to 30 min, can all kill the various microorganisms on the pot and their spores or spores. High pressure steam sterilization is wide, high efficiency is the most commonly used sterilization process in microbiology experiments.