Autocalve steam sterilization is to be sterilized items in a sealed pressure sterilization pot, by heating, sterilization pot every suites boil water and make steam, for steam rapidly will drive the pot of cold air from the vent valve, then close vent valve, continue to heat, at this time due to the steam can’t overflow, and increased the pressure inside the sterilizer, which makes higher boiling point, You get a temperature above 100 degrees Celsius. Causes the bacterial body protein coagulation denaturation to achieve the purpose of sterilization. High pressure and high heat sterilization, its strong bactericidal force, high efficiency, not only can kill the general bacteria, has a strong resistance to the bacterial spores. It is the most ideal and effective sterilization method in the physical sterilization method.
Autoclave is one of the most important sterilization equipment. Autoclave steam sterilization has the advantages of fast sterilization speed, reliable effect, high temperature, strong penetration, etc., improper use can lead to the failure of sterilization.

What kinds of materials are sterilized in the autoclave?

The following is a list of acceptable and unacceptable materials for autoclaves, and while this is not a complete list, it gives you a good idea of what is acceptable:
AUTOCLAVE-COMPATIBLE MATERIALS
·Glass.
·Bedding.
·Stainless steel.
·Culture flasks.
·Biological research instruments.
·Media solutions.
·Waste.
·Pipette tips.
·Surgical instruments.
·Gloves.
Remember, putting an unacceptable material in your autoclave can cause damage, cost money in repairs and slow your production.
AUTOCLAVE-INCOMPATIBLE MATERIALS
·Chlorine.
·Bleach.
·Non-stainless steel.
·Salt/seawater.
·Low- or high-density polyethylene.
·Polyurethane.
·Liquids.
·Sulfates.
·Chlorides.
NEVER AUTOCLAVE:
· Flammable, reactive, corrosive, toxic or radioactive materials
· Household bleach
· Any liquid in a sealed container.
· Any material contained in such a manner that it touches the interior surfaces of the autoclave.
· Paraffin-embedded tissue.